bio rad nlc sensor chip  (Bio-Rad)

Journal: Immunity

Article Title: Viral DNA Binding to NLRC3, an Inhibitory Nucleic Acid Sensor, Unleashes STING, a Cyclic Dinucleotide Receptor that Activates Type I Interferon

doi: 10.1016/j.immuni.2019.02.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Anti-NLRC3 Santa Cruz Cat#sc-398947 Rabbit Anti-HA Cell Signaling Cat#3724; RRID: AB_1549585 Rabbit Anti-Flag Cell Signaling Cat#14793; RRID: AB_2572291 Mouse Anti-Halo Promega Cat#G9211; RRID: AB_2688011 Mouse Anti-His GenScript Cat#A00186-100; RRID: AB_914704 Mouse anti-HA Sigma Cat#H3663; RRID: AB_262051 Rabbit anti-Flag Sigma Cat#F7425; RRID: AB_439687 Goat anti-mouse IgG Invitrogen Cat#A-11003; RRID: AB_141370 Goat-anti-rabbit IgG Invitrogen Cat#A-11008; RRID: AB_143165 Chemicals DMEM GIBCO Cat#12430-05 Fetal Bovine Serum SigmaAldrich Cat#F7305 β-mercaptoethanol SigmaAldrich Cat#M6250 TRIzol Thermofisher Cat#1559602 SYBR Green Master Mix Thermofisher Cat#4344463 NP-40 lysis buffer Boston BioProducts Cat#BP-119 EDTA-free Protease inhibitor Roche Cat#11873580001 Streptavidin Sepharose beads Sigma Cat#GE17-5113-01 Mouse Anti-HA agarose beads Sigma Cat#E6779 Polybrene Sigma Cat#H9268 Puromycin Sigma Cat#P8833 G418 Thermofisher Cat#10131035 Doxycycline hyclate Sigma Cat#D9891 Lipofectamine2000 Thermofisher Cat#11668019 Aqua Polymount Polysciences Cat#18606-20 6% Novex DNA Retardation gel Invitrogen Cat#EC6365BOX Nylon membrane Schleicher & Schuell Cat#32-10416216 CHAPS Thermo Fisher Cat#28299 Streptavidin Magnetic Particles Roche Cat#11641778001 ProteOn NLC sensor chip Bio-Rad Cat#1765021 SureBeads Protein G Magnetic beads Bio-Rad Cat#161-4021 Proteinase K-Ambion Thermo Fisher Cat#AM2546 Mouse IL-1 beta Antibody R&D systems Cat#AF-401-NA Critical Commercial Assays Phusion Site-Directed Mutagenesis Kit Thermofisher Cat#F541 Lighshift Chemiluminescent EMSA kit Therofisher Cat#20148 Mouse IL-1β ELISA MAX Standard BioLegend Cat#432601 Recombinant DNA pcDNA3.1-HA-NLRC3 This paper N/A pcDNA3.1-HA-NLRX1 This paper N/A pcDNA3.1-HA-STING This paper N/A pcDNA3.1-Flag-NLRC3 This paper N/A pcDNA3.1-Flag-STING This paper N/A pcDNA3.1-Flag-RIG-I This paper N/A pcDNA3.1-HA-NLRC3ΔLRR1-4 This paper N/A pcDNA3.1-HA-NLRC3ΔLRR5-8 This paper N/A pcDNA3.1-HA-NLRC3ΔLRR9-12 This paper N/A pCDNA3.1-HA-NLRC3ΔLRR13-16 This paper N/A pCIG2-PURO-hNLRC3-3×Flag This paper N/A pCIG2-IRES-PURO Petrucelli et al., 2012 N/A pIndcer20-NLRC3-3×Flag This paper N/A pUNO1-hcGAS-Flag Provided by Dr. Blossom Damania N/A pCW-hSTING-HA Provided by Dr. Stanley Lemon N/A pET-28(a)-mouse cGAS This paper N/A pET-28(a)-human STING255-341 Provided by Dr. Pingwei Li N/A pDB-His-MBP-hman ASC1-106 Provided by Dr. Joseph A. Duncan N/A pcDNA3.1-HA-DDX41 Provided by Dr. Yongjun Liu N/A pcDNA3.1-HA-IFI16 Provided by Dr. Yongjun Liu N/A Experimental Models: Cell Lines HEK293T ATCC N/A HeLa ATCC N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J Jackson Lab Cat#000664 Mouse: B6.

Techniques: SYBR Green Assay, Lysis, Protease Inhibitor, Membrane, Magnetic Beads, Mutagenesis, Enzyme-linked Immunosorbent Assay, Recombinant, Software